Highly Sensitive Fluorescence Quantitative Detection of Mercury in Soil Based on Non-labeled Molecular Beacon and Fluorescent Dye Hoechst 33258
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چکیده
منابع مشابه
Measurement of cell numbers in microtiter culture plates using the fluorescent dye Hoechst 33258.
A sensitive fluorimetric assay based on DNA binding of the fluorescent dye Hoechst 33258 was developed to quantitate cell numbers in microtiter plates. The cells were fixed and incubated with the dye solution. The bound dye was extracted with ethanol and fluorescence was measured in a spectrofluorimeter. The method is sensitive for as little as a thousand cells and is particularly useful for th...
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The Hoechst dye fluorescence technique, which is used for the quantitative determination of DNA (12), requires high saline concentrations at neutral pH to reach maximal fluorescence of the DNA-dye complex and to render the DNA fully accessible to the dye by dissociating foreign molecules (3,12). In conventional assays, the DNA is alcohol-precipitated and then resuspended in buffer containing 2 ...
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پژوهش حاضر با هدف بررسی اثر جیره حاوی کربوهیدراتهای غیرالیافی زیاد و بروز التهاب در گاوهای شیرده در دو آزمایش جداگانه انجام شد. در آزمایش اول 20 رأس گاو مبتلا به ورم پستان از دو گروه کم تولید و پرتولید (هر کدام 10 رأس) و 20 رأس گاو سالم از دو گروه کم تولید و پرتولید (هر کدام 10 رأس) انتخاب شدند. همه گاوها در دوره دوم و یا سوم شیردهی و در روزهای 80-120 پس از زایش بودند. میانگین تولید شیر در گروه...
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ژورنال
عنوان ژورنال: Analytical Sciences
سال: 2017
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.33.275